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Transforming Growth Factor-β Induces Interleukin-6 Secretion From Human Ligamentum Flavum-Derived Cells Through Partial Activation Of P38 And P44/42 Mitogen-Activated Protein Kinases London Spine Lumbar Stenosis

The article presents the results of an experimental study on the intracellular signaling mechanism of interleukin-6 (IL-6) secretion in human ligamentum flavum-derived cells (HFCs) that were stimulated with transforming growth factor-β (TGF-β). Lumbar spinal stenosis (LSS) is a prevalent disease characterized by debilitating pain in the lower extremities, and its underlying pathomechanism remains unclear. The study found that TGF-β administration stimulated IL-6 release in a dose- and time-dependent manner. Inhibitors of p38 and p44/42 MAP kinases suppressed IL-6 secretion and gene expression in response to TGF-β stimulation. These findings suggest a potential association between IL-6-mediated inflammatory response and tissue hypertrophy in LSS, providing insights into molecular targets for therapeutic interventions targeting LSS-related inflammation

Summarised by Mr Mo Akmal – Lead Spinal Surgeon
The London Spine Unit : innovative day surgery unit in UK

Published article

CONCLUSIONS: Our findings indicate that TGF-β induces IL-6 protein secretion and gene expression in HFCs through the activation of p38 or p44/42 MAP kinases. These results suggest a potential association between IL-6-mediated inflammatory response and tissue hypertrophy in LSS, and we provide insights into molecular targets for therapeutic interventions targeting LSS-related inflammation through our analysis of the MAP kinase pathway using HFCs.

Spine Lumbar Spinal Stenosis Expert. Best Spinal Surgeon UK
Abstract Study design: This experimental study was performed using human ligamentum flavum-derived cells (HFCs). Purpose: To investigate the intracellular signaling mechanism of interleukin-6 (IL-6) secretion in transforming growth factor-β (TGF- β)-stimulated HFCs. Overview of literature: Lumbar spinal stenosis (LSS) is a prevalent disease among the elderly, characterized by debilitating pain in the lower extremities. Although,

Abstract

Study design: This experimental study was performed using human ligamentum flavum-derived cells (HFCs).

Purpose: To investigate the intracellular signaling mechanism of interleukin-6 (IL-6) secretion in transforming growth factor-β (TGF- β)-stimulated HFCs.

Overview of literature: Lumbar spinal stenosis (LSS) is a prevalent disease among the elderly, characterized by debilitating pain in the lower extremities. Although the number of patients with LSS has increased in recent years, the underlying pathomechanism remains unclear. Clinical examinations typically rely on magnetic resonance imaging to diagnose patients, revealing ligamentum flavum hypertrophy. Some studies have suggested an association between ligamentum flavum hypertrophy and inflammation/fibrosis, and expression of TGF-β and IL-6 has been observed in surgically obtained ligamentum flavum samples. However, direct evidence linking TGF-β and IL-6 expression in HFCs is lacking.

Methods: HFCs were obtained from patients with LSS who had undergone decompression surgery. The cells were stimulated with TGF-β and pretreated with either the p38 mitogen-activated protein (MAP) kinase inhibitor SB203580 or the p44/42 MAP kinase inhibitor FR180204. IL-6 secretion in the cell culture medium and IL-6 messenger RNA (mRNA) expression levels were analyzed using an enzyme-linked immunoassay and real-time polymerase chain reaction, respectively.

Results: TGF-β administration resulted in a dose- and time-dependent stimulation of IL-6 release. Treatment with SB203580 and FR180204 markedly suppressed TGF-β-induced IL-6 secretion from HFCs. Moreover, these inhibitors suppressed IL-6 mRNA expression in response to TGF-β stimulation.

Conclusions: Our findings indicate that TGF-β induces IL-6 protein secretion and gene expression in HFCs through the activation of p38 or p44/42 MAP kinases. These results suggest a potential association between IL-6-mediated inflammatory response and tissue hypertrophy in LSS, and we provide insights into molecular targets for therapeutic interventions targeting LSS-related inflammation through our analysis of the MAP kinase pathway using HFCs.

Keywords: Interleukin-6; Ligamentum flavum; MAP kinase; Spinal stenosis; Transforming growth factor-β.

The London Spine Unit : innovative day surgery unit in UK

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Transforming Growth Factor-β Induces Interleukin-6 Secretion from Human Ligamentum Flavum-Derived Cells through Partial Activation of p38 and p44/42 Mitogen-Activated Protein Kinases

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Abstract Study design: This experimental study was performed using human ligamentum flavum-derived cells (HFCs). Purpose: To investigate the intracellular signaling mechanism of interleukin-6 (IL-6) secretion in transforming growth factor-β (TGF- β)-stimulated HFCs. Overview of literature: Lumbar spinal stenosis (LSS) is a prevalent disease among the elderly, characterized by debilitating pain in the lower extremities. Although

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