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Tag: adult|article link|mouse cord|Spinal Cord|Transplantation

Ex vivo infection of human embryonic spinal cord neurons prior to transplantation into adult mouse cord.

By wp_zaman

Ex vivo infection of human embryonic spinal cord neurons prior to transplantation into adult mouse cord.

BMC Neurosci. 2010;11:65

Authors: Márton G, Tombácz D, Tóth JS, Szabó A, Boldogköi Z, Dénes A, Hornyák A, Nógrádi A

Genetically modified pseudorabies virus (Prv) proved suitable for the delivery of foreign genes to rodent embryonic neurons ex vivo and maintaining foreign gene expression after transplantation into spinal cord in our earlier study. The question arose of whether human embryonic neurons, which are known to be more resistant to Prv, could also be infected with a mutant Prv. Specifically, we investigated whether a mutant Prv with deleted ribonucleotide reductase and early protein 0 genes has the potential to deliver marker genes (gfp and beta-gal) into human embryonic spinal cord neurons and whether the infected neurons maintain expression after transplantation into adult mouse cord.

PMID: 20509957 [PubMed – indexed for MEDLINE]