Serum MicroRNAs Mirror Damage Severity in a Massive Animal Mannequin of Thoracic Spinal Wire Damage.
Sci Rep. 2017 05 03;7(1):1376
Authors: Tigchelaar S, Streijger F, Sinha S, Flibotte S, Manouchehri N, So Ok, Shortt Ok, Okon E, Rizzuto MA, Malenica I, Courtright-Lim A, Eisen A, Keuren-Jensen KV, Nislow C, Kwon BK
Therapeutic improvement for spinal twine damage is hindered by the issue in conducting scientific trials, which thus far have relied solely on useful end result measures for affected person enrollment, stratification, and analysis. Organic biomarkers that precisely classify damage severity and predict neurologic end result would symbolize a paradigm shift in the best way spinal twine damage scientific trials could possibly be performed. MicroRNAs have emerged as enticing biomarker candidates on account of their stability in organic fluids, their phylogenetic similarities, and their tissue specificity. Right here we characterised a porcine mannequin of spinal twine damage utilizing a mixed behavioural, histological, and molecular strategy. We carried out next-generation sequencing on microRNAs in serum samples collected earlier than damage after which at 1, three, and 5 days put up damage. We recognized 58, 21, 9, and seven altered miRNA after extreme, average, and gentle spinal twine damage, and SHAM surgical procedure, respectively. These information have been mixed with behavioural and histological evaluation. General miRNA expression at 1 and three days put up damage strongly correlates with end result measures at 12 weeks put up damage. The info offered right here point out that serum miRNAs are promising candidates as biomarkers for the analysis of damage severity for spinal twine damage or different types of traumatic, acute, neurologic damage.
PMID: 28469141 [PubMed – indexed for MEDLINE]