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Roles of monocyte chemotactic protein 1 and nuclear factor-?B in immune response to spinal tuberculosis in a New Zealand white rabbit model.

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Roles of monocyte chemotactic protein 1 and nuclear factor-?B in immune response to spinal tuberculosis in a New Zealand white rabbit model.

Braz J Med Biol Res. 2017 Feb 20;50(3):e5625

Authors: Guo XH, Bai Z, Qiang B, Bu FH, Zhao N

Abstract
This study aimed to explore the roles of monocyte chemotactic protein 1 (MCP-1) and nuclear factor kappa B (NF-?B) in immune response to spinal tuberculosis in a New Zealand white rabbit model. Forty-eight New Zealand white rabbits were collected and divided into four groups: experimental group (n=30, spinal tuberculosis model was established), the sham group (n=15, sham operation was performed) and the blank group (n=3). The qRT-PCR assay and western blotting were applied to detect the mRNA and protein expressions of MCP-1 and NF-?B in peripheral blood. ELISA was used to measure serum levels of MCP-1, NF-?B, IFN-?, IL-2, IL-4, and IL-10. Flow cytometry was adopted to assess the distributions of CD4+, CD8+ lymphocytes and CD4+ CD25+ Foxp3 lymphocyte subsets. Compared with the sham and blank groups, the mRNA and protein expressions of MCP-1 and NF-?B in the experimental group were significantly increased. The experimental group had lower serum levels of IL-2 and IFN-? and higher serum level of IL-10 than the sham and blank groups. In comparison to the sham and blank groups, CD4+ T lymphocyte subsets percentage, CD4+/CD8+ ratio and CD4+ CD25+ Foxp3+ Tregs subsets accounting for CD4+ lymphocyte in the experimental group were lower, while percentage of CD8+ T lymphocyte subsets was higher. Our study provided evidence that higher expression of MCP-1 and NF-?B may be associated with decreased immune function of spinal tuberculosis, which can provide a new treatment direction for spinal tuberculosis.

PMID: 28225889 [PubMed – indexed for MEDLINE]

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