Neuroprotective Results of Valproic Acid in a Rat Mannequin of Cauda Equina Harm.
World Neurosurg. 2017 Dec;108:128-136
Authors: Kong QJ, Wang Y, Liu Y, Solar JC, Xu XM, Solar XF, Shi JG
BACKGROUND: Histone deacetylase inhibitors, together with valproic acid (VPA), are promising therapeutic interventions in neurological issues and play an vital function in synaptic exercise and neuronal operate.
METHODS: A complete of 30 rats have been randomly allotted to three teams: sham, management, and VPA. The rats within the VPA and management teams acquired laminectomy on the L4 degree of the vertebrae and silicone gel implantation into the epidural areas L5 and L6. Rats within the sham group solely acquired laminectomy on the L4 degree of vertebrae with none implantation. VPA (300 mg/kg in saline) was administered 2 hours earlier than the surgical procedure. After the surgical procedure, the VPA group acquired additional VPA injections at 300 mg/kg twice a day for 1 week. The identical quantity of saline was injected within the management group. Neurobehavioral assessments utilizing the Basso, Beattie, Bresnahan scale and the indirect board check have been carried out for 1 week beginning at 2 hours earlier than surgical procedure as much as day 7 after surgical procedure. At day 7 after surgical procedure, tissues from the compressed cauda equina (L5-L6) have been subjected to hematoxylin and eosin, luxol quick blue, or immunofluorescence staining, whereas the terminal deoxynucleotidyl transferase-mediated biotinylated UTP nick-end label assay staining was carried out on the tissue from the dorsal root ganglions and the lumbar phase of the spinal wire proximal to the compressed cauda equina (L5-L6).
RESULTS: The behavioral outcomes instructed a major enchancment within the decrease limb motor operate within the VPA group in contrast with controls (P < zero.05). Moreover, histologic evaluation revealed a major discount in nerve fibers exhibiting Wallerian degeneration and demyelinating lesions within the VPA group, along with an elevated myelination in contrast with the management group (P < zero.05). The terminal deoxynucleotidyl transferase-mediated biotinylated UTP nick-end label assay staining revealed a major lower within the variety of apoptotic neurons within the spinal wire anterior horn and dorsal root ganglions within the VPA group in contrast with controls (P < zero.05).
CONCLUSIONS: Our knowledge demonstrated that VPA may alleviate cauda equina harm, cut back apoptotic cells, and enhance motor restoration, suggesting a neuroprotective impact in acute cauda equina syndrome.
PMID: 28867325 [PubMed – indexed for MEDLINE]