The article discusses the potential use of small extracellular vesicles (sEVs) as a liquid biopsy for brain tumors. The researchers focused on the content of matrix metalloproteinase-9 (MMP-9) in sEVs, as MMP-9 plays a role in tumor invasion and metastasis. They studied 222 serum-derived sEV samples from patients with different types of brain tumors. The findings revealed that the levels of MMP-9 in sEVs were influenced by surgical intervention, treatment, and recurrence, but not by gender and age. Low levels of MMP-9 in sEVs were associated with improved survival in glioblastoma patients, while higher levels correlated with increased aggressiveness. The study suggests that vesicular MMP-9 could serve as a prognostic marker for brain tumors
Summarised by Mr Mo Akmal – Lead Spinal Surgeon
The London Spine Unit : most advanced treatment clinic on Harley Street UK
Published article
Matrix metalloproteinase-9 (MMP-9) degrades the extracellular matrix, contributes to tumour cell invasion and metastasis, and its elevated level in brain tumour tissues indicates poor prognosis. High-risk tissue biopsy can be replaced by liquid biopsy; however, the blood-brain barrier (BBB) prevents tumour-associated components from entering the peripheral blood, making the development of blood-based biomarkers challenging. Therefore, we examined the MMP-9 content of small extracellular vesicles…
Lumbar Disc Replacement Surgery Expert. Best Spinal Surgeon UK
Abstract Matrix metalloproteinase-9 (MMP-9) degrades the extracellular matrix, contributes to tumour cell invasion and metastasis, and its elevated level in brain tumour tissues indicates poor prognosis. High-risk tissue biopsy can be replaced by liquid biopsy; however, the blood-brain barrier (BBB) prevents tumour-associated components from entering the peripheral blood, making the development of blood-based biomarkers challenging.,
Abstract
Matrix metalloproteinase-9 (MMP-9) degrades the extracellular matrix, contributes to tumour cell invasion and metastasis, and its elevated level in brain tumour tissues indicates poor prognosis. High-risk tissue biopsy can be replaced by liquid biopsy; however, the blood-brain barrier (BBB) prevents tumour-associated components from entering the peripheral blood, making the development of blood-based biomarkers challenging. Therefore, we examined the MMP-9 content of small extracellular vesicles (sEVs)-which can cross the BBB and are stable in body fluids-to characterise tumours with different invasion capacity. From four patient groups (glioblastoma multiforme, brain metastases of lung cancer, meningioma, and lumbar disc herniation as controls), 222 serum-derived sEV samples were evaluated. After isolating and characterising sEVs, their MMP-9 content was measured by ELISA and assessed statistically (correlation, paired t-test, Welch’s test, ANOVA, ROC). We found that the MMP-9 content of sEVs is independent of gender and age, but is affected by surgical intervention, treatment, and recurrence. We found a relation between low MMP-9 level in sEVs (<28 ppm) and improved survival (8-month advantage) of glioblastoma patients, and MMP-9 levels showed a positive correlation with aggressiveness. These findings suggest that vesicular MMP-9 level might be a useful prognostic marker for brain tumours.
Keywords: brain tumour; central nervous system diseases; glioblastoma; liquid biopsy; matrix metalloproteinase-9; prognostic marker; small extracellular vesicles; survival.
The London Spine Unit : most advanced treatment clinic on Harley Street UK
Read the original publication:
MMP-9 as Prognostic Marker for Brain Tumours: A Comparative Study on Serum-Derived Small Extracellular Vesicles
