MiR-210 facilitates ECM degradation by suppressing autophagy through silencing of ATG7 in human degenerated NP cells.
Biomed Pharmacother. 2017 Sep;93:470-479
Authors: Wang C, Zhang ZZ, Yang W, Ouyang ZH, Xue JB, Li XL, Zhang J, Chen WK, Yan YG, Wang WJ
Intervertebral disc degeneration (IDD) is considered the commonest reason behind low again ache. Dysregulation of microRNAs (miRNAs) is concerned within the growth of IDD. The intention of this examine was to discover the affect of miR-210 on sort II collagen (Col II) and aggrecan expression and attainable mechanisms in human degenerated nucleus pulposus (NP) cells. Our outcomes confirmed that miR-210 ranges have been considerably elevated in degenerated NP tissues in contrast with wholesome controls, and positively correlated with disc degeneration grade. By gain-of-function and loss-of-function research in human degenerated NP cells, miR-210 was proven to inhibit autophagy after which upregulate MMP-Three and MMP-13 expression, resulting in elevated degradation of Col II and aggrecan. Autophagy-related gene 7 (ATG7) was recognized as a direct goal of miR-210. Knockdown of ATG7 by small interfering RNA (siRNA) abrogated the consequences of miR-210 inhibitor on MMP-Three, MMP-13, Col II and aggrecan expression. Taken collectively, these outcomes recommend that miR-210 inhibits autophagy through silencing of ATG7, resulting in elevated Col II and aggrecan degradation in human degenerated NP cells.
PMID: 28667916 [PubMed – indexed for MEDLINE]