miR?15a represses most cancers cell migration and invasion beneath circumstances of hypoxia by focusing on and downregulating Bcl?2 expression in human osteosarcoma cells.
Int J Oncol. 2018 Feb 23;:
Authors: Leng J, Track Q, Zhao Y, Wang Z
Osteosarcoma is a typical, high-risk main bone malignancy that principally impacts the youthful inhabitants. There was no marked enchancment within the medical outcomes of osteosarcoma sufferers so far, and most cancers recurrence and metastasis are frequent in high-grade osteosarcoma. Due to this fact, figuring out new biomarkers and novel therapeutic targets is essential for enhancing the prognosis of osteosarcoma sufferers. Within the current examine, the MG63 human osteosarcoma cell line was employed to look at the function of microRNA (miR)?15a in regulating mobile actions beneath hypoxic circumstances. It was demonstrated that hypoxia stimulates migration and invasion in MG63 cells, which was correlated with the downregulation of miR?15a and upregulation of B-cell lymphoma 2 (Bcl?2) expression. Introduction of miR?15a or knockdown of endogenous Bcl?2 might cut back hypoxia-induced cell invasion and migration by means of the regulation of matrix metalloproteinases. Evaluation of the expression of miR?15a indicated that hypoxia repressed the transcription of deleted in lymphocytic leukemia 2 (DLEU2), which is the host gene of miR?15a. These findings indicated that miR?15a could also be a useful goal for the remedy of osteosarcoma, notably for sufferers with high-grade most cancers or heavy tumor burden.
PMID: 29484432 [PubMed – as supplied by publisher]