Magnetic Resonance Imaging-Guided Transplantation of Neural Stem Cells into the Porcine Spinal Wire.
Stereotact Funct Neurosurg. 2017;95(1):60-68
Authors: Lamanna JJ, Urquia LN, Hurtig CV, Gutierrez J, Anderson C, Piferi P, Federici T, Oshinski JN, Boulis NM
BACKGROUND: Cell-based therapies are a promising remedy possibility for traumatic, tumorigenic and degenerative ailments of the spinal wire. Transplantation into the spinal wire is achieved with intravascular, intrathecal, or direct intraparenchymal injection. The present customary for direct injection is proscribed by surgical invasiveness, issue in reinjection, and the lack to instantly goal anatomical or pathological landmarks. The target of this research was to current the proof of precept for minimally invasive, percutaneous transplantation of stem cells into the spinal wire parenchyma of reside minipigs underneath MR steering.
METHODS: An MR-compatible backbone injection platform was developed to work with the ClearPoint SmartFrame system (MRI Interventions Inc.). The system was connected to the backbone of two reside minipigs, a percutaneous injection cannula was superior into the spinal wire underneath MR steering, and cells have been delivered to the wire.
RESULTS: A graft of two.5 × 106 human (n = 1) or porcine (n = 1) neural stem cells labeled with ferumoxytol nanoparticles was transplanted into the ventral horn of the spinal wire with MR steering in 2 animals. Graft supply was visualized with postprocedure MRI, and attribute iron precipitates have been recognized within the spinal wire by Prussian blue histochemistry. Grafted stem cells have been noticed within the spinal wire of the pig injected with porcine neural stem cells. No postoperative morbidity was noticed in both animal.
CONCLUSION: This report helps the proof of precept for transplantation and visualization of pharmacological or organic brokers into the spinal wire of a big animal underneath the steering of MRI.
PMID: 28132063 [PubMed – indexed for MEDLINE]