In vivo imaging of white matter is important for the mechanistic understanding of demyelination and evaluation of remyelination therapies. Although white matter can be visualized by a strong coherent anti-Stokes Raman scattering (CARS) signal from axonal myelin, in vivo repetitive CARS imaging of the spinal cord remains a challenge due to complexities induced by the laminectomy surgery. We present a careful experimental design that enabled longitudinal CARS imaging of de- and remyelination at single axon level in live rats. In vivo CARS imaging of secretory phospholipase A(2) induced myelin vesiculation, macrophage uptake of myelin debris, and spontaneous remyelination by Schwann cells are sequentially monitored over a 3 week period. Longitudinal visualization of de- and remyelination at a single axon level provides a novel platform for rational design of therapies aimed at promoting myelin plasticity and repair
Keywords : Animals,Biomedical Engineering,Demyelinating Diseases,diagnostic imaging,Laminectomy,methods,Myelin Sheath,Optical Phenomena,pathology,Rats,Rats,Long-Evans,Rats,Sprague-Dawley,Schwann Cells,Spectrum Analysis,Raman,Spinal Cord,Spinal Cord Injuries,surgery,therapy,Time Factors,Universities,, Vivo,Coherent,Antistokes,Raman, skull trigger points
Date of Publication : 2011 Oct
Authors : Shi Y;Zhang D;Huff TB;Wang X;Shi R;Xu XM;Cheng JX;
Organisation : Purdue University, Weldon School of Biomedical Engineering, West Lafayette, Indiana 47907, USA
Journal of Publication : J Biomed Opt
Pubmed Link : https://www.ncbi.nlm.nih.gov/pubmed/22029359
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