A novel conotoxin named lt6c, an O-superfamily conotoxin, was identified from the cDNA library of venom duct of Conus litteratus. The full-length cDNA contains an open reading frame encoding a predicted 22-residue signal peptide, a 22-residue proregion and a mature peptide of 28 amino acids. The signal peptide sequence of lt6c is highly conserved in O-superfamily conotoxins and the mature peptide consists of six cysteines arranged in the pattern of C-C-CC-C-C that is defined the O-superfamily of conotoxins. The mature peptide fused with thioredoxin, 6-His tag, and a Factor Xa cleavage site was successfully expressed in Escherichia coli. About 12 mg lt6c was purified from 1L culture. Under whole-cell patch-clamp mode, lt6c inhibited sodium currents on adult rat dorsal root ganglion neurons. Therefore, lt6c is a novel O-superfamily conotoxin that is able to block sodium channels
Keywords : Adult,Amino Acid Sequence,Animals,Base Sequence,biosynthesis,Cells,Cultured,chemistry,China,Conotoxins,Conus Snail,Cysteine,drug effects,Escherichia coli,Female,genetics,isolation & purification,Male,metabolism,Molecular Sequence Data,Multigene Family,Neurons,pharmacology,physiology,Rats,Recombinant Proteins,Sequence Analysis,DNA,Sodium Channels,, Characterization,Novel, neck specialist near me
Date of Publication : 2008 Oct
Authors : Wang L;Pi C;Liu J;Chen S;Peng C;Sun D;Zhou M;Xiang H;Ren Z;Xu A;
Organisation : State Key Laboratory of Biocontrol, Guangdong Province Key Laboratory of Pharmaceutical Functional Genes, Department of Biochemistry, College of Life Sciences, Sun Yat-sen (Zhongshan) University, Guangzhou, 510275, People’s Republic of China
Journal of Publication : J Pept Sci
Pubmed Link : https://www.ncbi.nlm.nih.gov/pubmed/18523965
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