19 Harley St, London, W1G 9QJ, UK
We are London's Top Spine Clinic

Hydrogel-Assisted Antisense LNA Gapmer Supply for In Situ Gene Silencing in Spinal Twine Damage.

Related Articles

Hydrogel-Assisted Antisense LNA Gapmer Supply for In Situ Gene Silencing in Spinal Twine Damage.

Mol Ther Nucleic Acids. 2018 Jun 01;11:393-406

Authors: Moreno PMD, Ferreira AR, Salvador D, Rodrigues MT, Torrado M, Carvalho ED, Tedebark U, Sousa MM, Amaral IF, Wengel J, Pêgo AP

After spinal wire damage (SCI), nerve regeneration is severely hampered as a result of institution of a extremely inhibitory microenvironment on the damage web site, via the contribution of a number of elements. The potential of antisense oligonucleotides (AONs) to change gene expression at totally different ranges, permitting the regulation of cell survival and cell operate, along with the supply of chemically modified nucleic acids with favorable biopharmaceutical properties, make AONs a horny device for novel SCI remedy developments. On this work, we explored the potential of locked nucleic acid (LNA)-modified AON gapmers together with a fibrin hydrogel bridging materials to induce gene silencing in situ at a SCI lesion web site. LNA gapmers have been successfully developed towards two promising gene targets aiming at enhancing axonal regeneration-RhoA and GSK3?. The fibrin-matrix-assisted AON supply system mediated potent RNA knockdown in vitro in a dorsal root ganglion explant tradition system and in vivo at a SCI lesion web site, reaching round 75% downregulation 5 days after hydrogel injection. Our outcomes present that native implantation of a AON-gapmer-loaded hydrogel matrix mediated environment friendly gene silencing within the lesioned spinal wire and is an progressive platform that may probably mix gene regulation with regenerative permissive substrates aiming at SCI therapeutics and nerve regeneration.

PMID: 29858074 [PubMed]

Share to care...

Share on facebook
Share on twitter
Share on pinterest
Share on google
Share on linkedin
Share on skype

What we do...

The Harley Street Hospital


What is London spine unit and How it Works

The London Spine Unit was established in 2005 and has successfully treated over 5000 patients. All conditions are treated.

We treat all spinal disorders

The London Spine Unit specialises in Minimally Invasive Treatments allowing rapid recovery and return to normal function

Trusted by patients worldwide

The London Spine Unit provides the highest quality care to all patients and has VIP services for those seeking exceptional services

What our patients say about us ......