19 Harley St, London, W1G 9QJ, UK

[High-performance liquid chromatography for detecting the concentrations of isoniazid and acetylisoniazid in spinal tuberculosis]

OBJECTIVE: To study the distribution of isoniazid and its metabolite in spinal tuberculosis following chemotherapy. METHODS: Twenty-three patients with spinal tuberculosis received chemotherapy with 2SHRZ/16HRZ (for a total of 18 months). Four weeks after the chemotherapy, all the patients underwent surgery and specimens of the serum, ilium and vertebral tissue including the sclerotic wall, focus inside the sclerotic wall (if present) and destructed foci were obtained. CT was performed in all the cases to test the HU of the foci before operation, and the levels of isoniazid and its metabolite in the specimen were measured with high-performance liquid chromatography (HPLC). RESULTS: The levels of isoniazid and its metabolite were the highest in the serum, followed by normal ilium and non-sclerotic bone, and were extremely low in the sclerotic wall and foci. Their levels in the non-sclerotic bone of the compromised vertebra and normal vertebra showed no significant difference (P>0.05), but in the sclerotic bone, their levels were significantly higher than in the normal vertebra (P<0.05). Isoniazid and its metabolite are hardly detectable in the sclerotic foci in the compromised vertebrae. CONCLUSION: Isoniazid and its metabolite may reach therapeutic concentration in normal vertebra and nonsclerotic bones of the compromised vertebra, but not in the disease foci or the sclerotic bone of the compromised vertebrae Keywords : Adult,Aged,analogs & derivatives,Antitubercular Agents,blood,China,Chromatography,High Pressure Liquid,drug therapy,Female,Humans,Ilium,Isoniazid,Lumbar Vertebrae,Male,metabolism,methods,Middle Aged,pharmacokinetics,surgery,therapeutic use,Tuberculosis,Tuberculosis,Spinal,Young Adult,, Liquid,Chromatography,Detecting, zydol side effects

Date of Publication : 2010 Feb

Authors : Liu P;Jiang JM;Zhou ZF;

Organisation : Department of Orthopedic Surgery, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. boy318@tom.com

Journal of Publication : Nan Fang Yi Ke Da Xue Xue Bao

Pubmed Link : https://www.ncbi.nlm.nih.gov/pubmed/20159724

The London Spine Unit : Harley Street UK. Specialists in Cutting Edge Technologies for Spinal Surgery

Make an Appointment 

Trustpilot Reviews
Doctify Reviews
Top Doctor Reviews

High-performance liquid chromatography for detecting the concentrations of isoniazid and acetylisoniazid in spinal tuberculosis | Claudication pain

What our patients say ...

Consultant Spine Surgeon
Consultant Spine Surgeon
Consultant Spine Surgeon

This surgical technique consists of a percutaneous approach for the treatment of small to medium size hernias of the intervertebral disc by laser energy. The main objective is to reduce the intradiscal pressure in the nucleus pulposus

Laser Disc Surgery can be performed under local anaesthetic as a day case at our centre on the prestigious Harley Street.
What is London spine unit and How it Works

The London Spine Unit was established in 2005 and has successfully treated over 5000 patients. All conditions are treated.

treatment of all spinal disorders

The London Spine Unit specialises in Minimally Invasive Treatments allowing rapid recovery and return to normal function

Trusted by patients worldwide

The London Spine Unit provides the highest quality care to all patients and has VIP services for those seeking exceptional services

If you have any emergency Doctor’s need, simply call our 24 hour emergency

Your personal case manager will ensure that you receive the best possible care.

Call Now 

+44 844 589 2020
+44 203 973 8810