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Results of human parathyroid hormone 1-34 on bone loss and lumbar intervertebral disc degeneration in ovariectomized rats.
Int Orthop. 2018 Feb 13;:
Authors: Luo Y, Li SY, Tian FM, Music HP, Zhang YZ, Zhang L
Summary
PURPOSE: Lumbar intervertebral disc degeneration is a typical explanation for decrease again ache that impacts the bodily and psychological well being of sufferers and will increase social burden. Parathyroid hormone has been reported to be efficient at inhibiting disc degeneration; nonetheless, these results haven’t been absolutely established in vivo in ovariectomized (OVX) rats. Thus, on this examine, we aimed to deal with this challenge and look at the consequences of parathyroid hormone therapy in OVX rats.
METHODS: Thirty feminine Sprague-Dawley rats, three months-old, have been subjected to sham or ovariectomy surgical procedure. Twelve weeks postsurgery, OVX rats have been handled with both human parathyroid hormone [hPTH(1-34), 30 ?g/kg/day] or automobile (regular saline (NS)) therapy. The L3-6 spinal segments have been harvested after 12 weeks therapy. Bone mineral density (BMD), micro-architectural parameters, and biomechanical evaluation have been measured on the lumbar vertebral our bodies. Histology and immunohistochemistry have been carried out to investigate the traits of the lumbar intervertebral discs.
RESULTS: OVX + PTH rats had considerably greater BMD, share bone quantity density, trabecular thickness, and biomechanical energy in contrast with these in Sham and OVX + NS rats. Histology and immunostaining revealed that disc degeneration was not considerably totally different between the OVX + NS rats and the OVX + PTH rats, in contrast with the Sham group; the construction of nucleus pulposus was disordered, the expression of collagen I used to be elevated, and collagen II and aggrecan have been decreased.
CONCLUSIONS: These findings confirmed that hPTH(1-34) therapy has substantial anabolic results on bone mass and trabecular micro-architecture, whereas the excessively enhanced bone mass and energy have been coupled with a non-significant impact on the disc degeneration in ovariectomized rats.
PMID: 29442158 [PubMed – as supplied by publisher]