Live Video Consultation
19 Harley St, London, W1G 9QJ, UK

[Effects of bone marrow mesenchymal stem cells transplantation on expression of vascular endothelial growth factor gene and angiogenesis after spinal cord injury in rats]

OBJECTIVE: To investigate the effect of bone marrow mesenchymal stem cells (BMSCs) transplantation on the motor function recovery, the expression of vascular endothelial growth factor (VEGF) gene, and angiogenesis after spinal cord injury (SCI) in rats, and to explore the treatment mechanism of BMSCs in SCI. METHODS: BMSCs were isolated and cultured from the marrow of 5 Wistar rats (4 weeks old) and the 3rd-4th passage cells were prepared for the experiment. A total of 87 adult female Wistar rats (weighing 220-250 g) were randomly divided into 3 groups: sham-operated group (group A, n=21), DMEM group (group B, n=33), BMSCs group (group C, n=33). A laminectomy was only performed at T8-10 levels in group A. The SCI models were established by modified Nystrom’s compression method in groups B and C, and BMSCs and DMEM were injected in groups B and C respectively at 30 minutes after SCI. Basso-Beattie-Bresnahan (BBB) score was used for the motor function recovery at 3, 7, 14, and 28 days, RT-PCR for the VEGF mRNA at 1, 3, and 5 days, and immunohistochemical staining for angiogenesis at 3, 7, 14, and 28 days. RESULTS: In groups B and C, the hindlimb locomotor function was improved at different degrees with time, showing significant difference in BBB score between groups B, C and group A (P < 0.05). At 28 days, the BBB score in group C was significantly higher than that in group B (P < 0.05) and there was no significant difference between groups B and C (P > 0.05) at 3, 7, and 14 days after transplantation. The numbers of microvessels in the ventral horns of gray matter around SCI in groups B and C were significantly lower than that in group C (P < 0.05) at 3 days, but there was no significant difference at 7, 14, and 28 days after transplantation (P > 0.05). There was no significant difference in the number of microvessels between group C and group B (P > 0.05) at 3 and 7 days, but the number of microvessels in group C was significantly higher than that in group B (P < 0.05) at 14 and 28 days after transplantation. However, there was no significant difference in the number of microvessels in the white matter around SCI in 3 groups at different time points after transplantation (P > 0.05). The RT-PCR results showed that VEGF mRNA expressed at a low level in group A. Compared with group A, the expression level of VEGF mRNA in groups B and C increased at 1 day and reached the peak at 3 days, then decreased at 5 days after transplantation; and the expression of VEGF mRNA was significantly higher in groups B and C than in group A (P < 0.05), and in group C than in group B (P < 0.05) at 1, 3, and 5 days. CONCLUSION: BMSCs may promote the motor function recovery by up-regulating VEGF mRNA expression and increasing angiogenesis in the spinal cord after SCI in rats Keywords : Adult,Animals,Bone Marrow,Bone Marrow Cells,cytology,Female,Gene Expression,genetics,Growth,Hindlimb,injuries,Laminectomy,Mesenchymal Stem Cell Transplantation,Mesenchymal Stem Cells,metabolism,methods,Neovascularization,Physiologic,Orthopedics,pathology,Rats,Rats,Wistar,Spinal Cord,Spinal Cord Injuries,Stem Cells,surgery,Time,transplantation,Universities,Vascular Endothelial Growth Factor A,, Bone,Marrow,Mesenchymal,Stem, fibromyalgia pain management centers

Date of Publication : 2011 Jul

Authors : Yu D;Lu G;Cao Y;Li G;Zhi X;Fan Z;

Organisation : Department of Orthopedics, the First Affiliated Hospital, Liaoning Medical University, Jinzhou Liaoning, 121000, P.R.China

Journal of Publication : Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi

Pubmed Link : https://www.ncbi.nlm.nih.gov/pubmed/21818951

The London Spine Unit : Harley Street UK. Specialists in Cutting Edge Technologies for Spinal Surgery

Make an Appointment 

Trustpilot Reviews
Doctify Reviews
Top Doctor Reviews

Effects of bone marrow mesenchymal stem cells transplantation on expression of vascular endothelial growth factor gene and angiogenesis after spinal cord injury in rats | Cymbalta for pain and depression

What our patients say ...

Consultant Spine Surgeon
Consultant Spine Surgeon
Consultant Spine Surgeon

This surgical technique consists of a percutaneous approach for the treatment of small to medium size hernias of the intervertebral disc by laser energy. The main objective is to reduce the intradiscal pressure in the nucleus pulposus

Laser Disc Surgery can be performed under local anaesthetic as a day case at our centre on the prestigious Harley Street.
What is London spine unit and How it Works

The London Spine Unit was established in 2005 and has successfully treated over 5000 patients. All conditions are treated.

treatment of all spinal disorders

The London Spine Unit specialises in Minimally Invasive Treatments allowing rapid recovery and return to normal function

Trusted by patients worldwide

The London Spine Unit provides the highest quality care to all patients and has VIP services for those seeking exceptional services

If you have any emergency Doctor’s need, simply call our 24 hour emergency

Your personal case manager will ensure that you receive the best possible care.

Call Now 

+44 844 589 2020
+44 203 973 8810