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Curcumin alleviates lumbar radiculopathy by lowering neuroinflammation, oxidative stress and nociceptive elements.
Eur Cell Mater. 2017 05 09;33:279-293
Authors: Xiao L, Ding M, Fernandez A, Zhao P, Jin L, Li X
Summary
Present non-surgical remedies for lumbar radiculopathy [e.g. epidural steroids and Tumour necrosis factor-? (TNF-?) antagonists] are neither efficient nor protected. As a non-toxic pure product, curcumin possesses an distinctive anti-inflammatory profile. We hypothesised that curcumin alleviates lumbar radiculopathy by attenuating neuroinflammation, oxidative stress and nociceptive elements. In a dorsal root ganglion (DRG) tradition, curcumin successfully inhibited TNF-?-induced neuroinflammation, in a dose-dependent method, as proven by mRNA and protein expression of IL-6 and COX-2. Such results could be mediated through protein kinase B (AKT) and extracellular sign regulated kinase (ERK) pathways. Additionally, an analogous impact in combating TNF-?-induced neuroinflammation was noticed in remoted major neurons. As well as, curcumin protected neurons from TNF-?-triggered extreme reactive oxygen species (ROS) manufacturing and mobile apoptosis and, accordingly, promoted mRNA expression of the anti-oxidative enzymes haem oxygenase-1, catalase and superoxide dismutase-2. Intriguingly, digital von Frey check prompt that intraperitoneal injection of curcumin considerably abolished ipsilateral hyperalgesia secondary to disc herniation in mice, for as much as 2 weeks post-surgery. Such in vivo ache alleviation might be attributed to the suppression, noticed in DRG explant tradition, of TNF-?-elicited neuropeptides, comparable to substance P and calcitonin gene-related peptide. Surprisingly, micro-computed tomography (?CT) knowledge prompt that curcumin therapy might promote disc peak restoration following disc herniation. Alcian blue/picrosirius pink staining confirmed that systemic curcumin administration promoted regeneration of extracellular matrix proteins, visualised by presence of plentiful newly-formed collagen and proteoglycan content material in herniated disc. Our research supplied pre-clinical proof for expediting this pure, non-toxic pleiotropic agent to turn into a brand new and protected medical therapy of radiculopathy.
PMID: 28485773 [PubMed – indexed for MEDLINE]
