Cryopreserved human umbilical twine versus biocellulose movie for prenatal spina bifida restore in a physiologic rat mannequin.
Prenat Diagn. 2017 Might;37(5):473-481
Authors: Snowise S, Mann L, Morales Y, Moise KJ, Johnson A, Fletcher S, Grill RJ, Tseng SCG, Papanna R
BACKGROUND: Prenatal spina bifida (SB) restore with a regenerative patch might enhance neurological outcomes by lowering inflammatory scarring.
OBJECTIVE: This examine goals to match cryopreserved human umbilical twine (HUC) and biocellulose movie (BCF) patches sutured over SB lesions for regeneration of native cells and inflammatory response.
STUDY DESIGN: Sprague-Dawley rats had been gavaged with retinoic acid (RA) on embryonic day 10 to induce SB. Hysterotomy was carried out on embryonic day 20 and on HUC or BCF patches sutured over the defect. Pups had been harvested 30 to 34 h later, and hematoxylin and eosin staining and Trichrome staining assessed fundamental mobile migration. Immunohistochemistry demonstrated the precise nature of the mobile migration. Patches and surrounding exudates had been evaluated with microscopy and cells quantified.
RESULTS: Histology confirmed mobile migration with all HUC patches in contrast with none with BCF patches. Epithelial cells had been famous migrating over the dorsal HUC floor, astrocytes had been famous alongside the HUC floor adjoining to the lesion, and endothelial cells had been famous throughout the HUC. HUC patches confirmed minimal inflammatory cells. Exudates surrounding the HUC patches had fewer inflammatory cells than exudates round BCF patches.
CONCLUSION: HUC promotes mobile migration of native cells with minimal inflammatory response in contrast with BCF. HUC often is the superior patch materials for prenatal SB restore. © 2017 John Wiley & Sons, Ltd.
PMID: 28295455 [PubMed – indexed for MEDLINE]