Circulating tumor cells mirror bone metastatic phenotype in prostate most cancers.
Oncotarget. 2018 Jun 29;9(50):29403-29413
Authors: Josefsson A, Larsson Okay, Månsson M, Björkman J, Rohlova E, Åhs D, Brisby H, Damber JE, Welén Okay
Circulating tumor cells (CTCs) are promising biomarkers in prostate most cancers (PC) as a result of they derive from main tumor and metastatic tissues. On this examine, we used quantitative real-time PCR (qPCR) to check the expression profiles of 41 PC-related genes between paired CTC and spinal column metastasis samples from 22 PC sufferers that underwent surgical procedure for spinal twine compression. We noticed good concordance between the gene expression profiles within the CTC and metastasis samples in a lot of the PC sufferers. Expression of 9 genes (AGR2, AKR1C3, AR, CDH1, FOLH1, HER2, KRT19, MDK, and SPINK1) confirmed a big correlation between the CTC and metastasis samples. Hierarchical clustering evaluation confirmed an identical grouping of PC sufferers based mostly on the expression of those 9 genes in each CTC and metastasis samples. Our findings exhibit that CTCs mirror gene expression patterns in tissue metastasis samples from PC sufferers. Though low detection frequency of sure genes is a limitation in CTCs, our outcomes point out the potential for CTC phenotyping as a device to enhance individualized remedy in metastatic prostate most cancers.
PMID: 30034626 [PubMed]