Characterization of the Antibody Response after Cervical Spinal Twine Damage.
J Neurotrauma. 2017 Mar 15;34(6):1209-1226
Authors: Ulndreaj A, Tzekou A, Mothe AJ, Siddiqui AM, Dragas R, Tator CH, Torlakovic EE, Fehlings MG
The immune system performs a crucial and complicated function within the pathobiology of spinal twine damage (SCI), exerting each helpful and detrimental results. Growing proof means that there are damage level-dependent variations within the immune response to SCI. Sufferers with traumatic SCI have elevated ranges of circulating autoantibodies in opposition to parts of the central nervous system, however the function of those antibodies in SCI outcomes stays unknown. In rodent fashions of mid-thoracic SCI, antibody-mediated autoimmunity seems to be detrimental to restoration. Nevertheless, whether or not autoantibodies in opposition to the spinal twine are generated following cervical SCI (cSCI), the most typical stage of damage in people, stays undetermined. To handle this information hole, we investigated the antibody responses following cSCI in a rat mannequin of damage. We discovered elevated immunoglobulin G (IgG) and IgM antibodies within the spinal twine within the subacute section of damage (2 weeks), however not in additional continual phases (10 and 20 weeks). At 2 weeks post-cSCI, antibodies have been detected on the damage epicenter and co-localized with the astroglial scar and neurons of the ventral horn. These elevated ranges of antibodies corresponded with enhanced activation of immune responses within the spleen. Larger counts of antibody-secreting cells have been noticed within the spleen of injured rats. Additional, elevated ranges of secreted IgG antibodies and enhanced proliferation of T-cells in splenocyte cultures from injured rats have been discovered. These findings recommend the potential growth of autoantibody responses following cSCI within the rat. The affect of the post-traumatic antibody responses on useful outcomes of cSCI is a crucial subject that requires additional investigation.
PMID: 27775474 [PubMed – indexed for MEDLINE]