Menu
Menu
19 Harley St, London, W1G 9QJ, UK
We are London's Top Spine Clinic

Bone mesenchymal stem cell-conditioned medium attenuates the effect of oxidative stress injury on NSCs by inhibiting the Notch1 signaling pathway.

Icon for Wiley Related Articles

Bone mesenchymal stem cell-conditioned medium attenuates the effect of oxidative stress injury on NSCs by inhibiting the Notch1 signaling pathway.

Cell Biol Int. 2019 Mar 06;:

Authors: Niu Y, Xia X, Song P, Fang H, Dong F, Tao H, Yang C, Shen C

Abstract
Numerous studies have demonstrated the therapeutic effect of bone mesenchymal stem cells on Spinal cord injury (SCI), especially on neural stem cells (NSCs). However, the predominant mechanisms of bone mesenchymal stem cell (BMSCs) are unclear. Recently, some researchers have found that paracrine signaling plays a key role in the therapeutic capacity of BMSCs and emphasized that the protective effect of BMSCs may be due to paracrine factors. In this study, we aimed to investigate the potential mechanisms of BMSCs recover function to NSCs.NSCs were identified by immunocytochemistry. The oxidative stress environment was simulated by H2O2(50µM, 100µM, 200µM) for 2h. The apoptotic rate of the NSCs was detected via flow cytometry. Lactate dehydrogenase (LDH), malondialdehyde (MDA) and superoxide dismutase (SOD) activity were evaluated via corresponding assay kits. Western blot were used to detect the expressions of Notch1, HES1, Caspase-3, cleave Caspase-3, Bax and Bcl-2. We found that H2O2 could significantly induce the apoptosis of NSCs, increase LDH, MDA levels and decrease SOD activity by activating the Notch1 signaling pathway. DAPT (the specific blocker of Notch1) and BMSC-CM could significantly prevent the apoptotic effect and oxidative stress injury on NSCs that were treated with H2O2. We also revealed that BMSC-CM could decrease the expression of Notch1, Hes1, cleave Caspase-3, Bax and increases the expression of Bcl-2 in NSCs which was induced by H2O2. These results have revealed that BMSC-CM can neutralize the effect against oxidative stress injury on the apoptosis of NSCs by inhibiting the Notch1 signaling pathway.

PMID: 30839137 [PubMed – as supplied by publisher]

Share to care...

Share on facebook
Facebook
Share on twitter
Twitter
Share on pinterest
Pinterest
Share on google
Google+
Share on linkedin
LinkedIn
Share on skype
Skype

What we do...

The Harley Street Hospital

Testimonials

What is London spine unit and How it Works

The London Spine Unit was established in 2005 and has successfully treated over 5000 patients. All conditions are treated.

We treat all spinal disorders

The London Spine Unit specialises in Minimally Invasive Treatments allowing rapid recovery and return to normal function

Trusted by patients worldwide

The London Spine Unit provides the highest quality care to all patients and has VIP services for those seeking exceptional services

What our patients say about us ......