Menu
Menu
19 Harley St, London, W1G 9QJ, UK
We are London's Top Spine Clinic

[Autophagy expression and its significance in chondrocytes from a degenerate model of human cervical endplate].

Related Articles

[Autophagy expression and its significance in chondrocytes from a degenerate model of human cervical endplate].

Zhonghua Yi Xue Za Zhi. 2013 Aug 20;93(31):2474-7

Authors: Xiong SL, Xu HG, Wang H, Zhang M, Yu YF, Zhang W, Tu CD, Zhao QL, Lü K, Zhong M, Zhang MY

Abstract
OBJECTIVE: To explore the autophagy expression and examine its significance in chondrocytes in a degenerate model of human cervical vertebrae endplate.
METHODS: Cartilage endplates were obtained from 48 hospitalized patients with cervical vertebral fracture or dislocation at our hospital between February 2012 to August 2012. They were divided into cervical spondylosis group with cervical spondylotic myelopathy (n = 31) and control group (n = 17).Endplate chondrocytes were isolated by enzyme digestion and cultured in vitro. The cells were stained with toluidine blue and hematoxylin and eosin; laser scanning confocal microscope and monodansyl cadaverine (MDC) were used to observe autophagy in endplate chondrocytes; reverse transcription-polymerase chain reaction (RT-PCR) was used to detect the mRNA expression of typeIIcollagen and aggrecan and Western blot for the protein of LC3.
RESULTS: A degenerative cell model of human cervical endplate chondrocytes was established successfully in vitro. Compared with the common group, the cellular morphologies of degenerative group showed spindle changes. Autophagic body was stained with MDC.Intracellular and perinuclear LC3 protein was detected by laser confocal microscopy. Compared with the control group, the mRNA expressions of aggrecan (0.715 ± 0.194) and typeII collagen (0.628 ± 0.254) markedly decreased (0.845 ± 0.186,0.913 ± 0.254, P < 0.05) and LC3-II/LC3-Ideclined in cervical spondylosis group.
CONCLUSION: Autophagy plays an important pathogenic role in the process of human cervical disc degeneration. And regulating its expression may improve disc degeneration in endplate cartilage cells.

PMID: 24300268 [PubMed – in process]

Share to care...

Share on facebook
Facebook
Share on twitter
Twitter
Share on pinterest
Pinterest
Share on google
Google+
Share on linkedin
LinkedIn
Share on skype
Skype

What we do...

The Harley Street Hospital

Testimonials

What is London spine unit and How it Works

The London Spine Unit was established in 2005 and has successfully treated over 5000 patients. All conditions are treated.

We treat all spinal disorders

The London Spine Unit specialises in Minimally Invasive Treatments allowing rapid recovery and return to normal function

Trusted by patients worldwide

The London Spine Unit provides the highest quality care to all patients and has VIP services for those seeking exceptional services

What our patients say about us ......

Facet arthropathy patient

They were very professional with good communication between doctor and patient. I also received correspondence after each consultation. Oscar F. You May Also Like:Disc prolapsePatient

Read More »