Evaluation of Lengthy Noncoding RNA and mRNA Expression Profiles in IL-9-Activated Astrocytes and EAE Mice.
Cell Physiol Biochem. 2018;45(5):1986-1998
Authors: Liu X, Zhang Q, Wang W, Zuo D, Wang J, Zhou F, Niu L, Li X, Qin S, Kou Y, Kong F, Pan W, Wang Y, Gao D, Solar H, Meves JM, Zheng Okay, Tang R
BACKGROUND/AIMS: A number of sclerosis (MS) is an autoimmune illness within the central nervous system related to demyelination and axonal harm. Astrocyte activation is concerned within the pathogenesis of MS and experimental autoimmune encephalomyelitis (EAE), an animal mannequin of MS. This examine was designed to seek out potential lncRNAs in EAE mice and activated astrocytes.
METHODS: we carried out microarray evaluation of lncRNAs from the mind tissues of EAE mice and first mouse astrocytes handled with IL-9(50 ng/ml). 12 lncRNAs have been validated by way of real-time PCR. Gene ontology and KEGG pathway evaluation have been utilized to discover the potential features of lncRNAs.
RESULTS: Differentially expressed 3300 lncRNAs and 3250 mRNAs have been within the mind tissues of EAE mice, and 3748 lncRNAs and 3332 mRNAs have been in activated astrocytes. Notably, there have been 2 co-up-regulated lncRNAs and three co-down-regulated lncRNAs each within the mind tissues of EAE mice and in activated astrocytes, together with Gm14005, Gm12478, mouselincRNA1117, AK080435, and mouselincRNA0681, which regulate the ER calcium flux kinetics, zinc finger protein and cell apoptosis. Equally, there have been 7 mRNAs co-up-regulated and a pair of mRNAs co-down-regulated each in vivo and in vitro. Gene ontology and KEGG pathway evaluation confirmed that the organic features of differentially expressed mRNAs have been related to metabolism, improvement and irritation. The outcomes of realtime PCR validation have been per the info from the microarrays.
CONCLUSIONS: Our knowledge uncovered the expression profiles of lncRNAs and mRNAs in vivo and in vitro, which can assist delineate the mechanisms of astrocyte activation throughout MS/EAE course of.
PMID: 29518786 [PubMed – indexed for MEDLINE]