A New Strategy in Gene Remedy of Glioblastoma Multiforme: Human Olfactory Ensheathing Cells as a Novel Provider for Suicide Gene Supply.
Mol Neurobiol. 2016 Oct;53(eight):5118-28
Authors: Hashemi M, Fallah A, Aghayan HR, Arjmand B, Yazdani N, Verdi J, Ghodsi SM, Miri SM, Hadjighassem M
Olfactory ensheathing cells (OECs) of human olfactory mucosa are a kind of glial-like cells that possess good migratory and tropism properties. We imagine that neuronal-derived automobile could have higher functionality to obtain to the location of harm. Along with, acquiring of such automobile from the affected person reduces threat of undesirable problems. So, on this research, we examine whether or not human olfactory ensheathing cells can be utilized as a cell supply for the primary time in gene supply to assay the tumoricidal impact of herpes simplex virus thymidine kinase gene (HSV-tk) on glioblastoma multiforme (GBM). We obtained OECs from superior turbinate of human nasal cavity mucosa, and cell phenotype was confirmed by the expression of cell-specific antigens together with low-affinity nerve progress issue receptor (p75 neurotrophin receptor), microtubule-associated protein-2 (MAP2), and S100 calcium binding protein B (S100-beta) utilizing immunocytochemistry. Then, these cells have been transduced by lentiviral vector for transient and secure expression of the herpes simplex virus thymidine kinase gene (OEC-tk). The migratory capability of OEC-tk, their efficiency to transform prodrug ganciclovir to poisonous type, and cytotoxic impact on astrocyte cells have been assayed in vitro. The OECs confirmed fibroblast-like morphology and expressed particular antigens akin to p75 neurotrophin receptor, S100-beta, and MAP2. Our outcomes indicated that OECs-tk have been in a position to migrate towards main cultured human glioblastoma multiforme and affected survival price of tumor cells in line with publicity time and focus of ganciclovir. Additionally, OECs-HSV-tk was able to inducing apoptosis in tumor cells. Our findings recommend that human OECs might make use of as a potential device to switch anticancer agent in gene remedy of mind tumor.
PMID: 26395282 [PubMed – indexed for MEDLINE]