A model of acute compressive spinal cord injury with a minimally invasive balloon in goats.
J Neurol Sci. 2013 Nov 22;
Authors: Cao P, Zheng Y, Zheng T, Sun C, Lu J, Rickett T, Shi R
Research into spinal cord injury depends upon animal models of trauma. While investigations using small animals have yielded critical insights into the cellular mechanisms of neurotrauma, no effective therapies have been translated to human clinical treatments. There are considerable differences in pathophysiology, scale, and anatomical organization between rodents and primates. Here, the established method of inflating balloons to compress the cord within the spinal canal was adapted for use in goats. By using surgical techniques to insert a kyphoplasty balloon, spinal cord injury was accomplished with minimal trauma to the surrounding tissues, as is common in other traumatic models. Dye volumes of 0, 1.26±0.18, and 2.82±0.20mL were injected into the balloon to produce spinal occupancies of 0%, 33±2%, and 89±4%, as evaluated by X-ray and computerized tomography imaging. A significant dose response was observed for the different levels of trauma, with reduced conduction of somatosensory evoked potentials and impaired mobility 7days after injury. From the strong correlations between injection volume, balloon pressure, spinal occupancy, nerve function, and animal behavior, we conclude that hydraulic compression in goats is a useful model of spinal cord injury.
PMID: 24332594 [PubMed – as supplied by publisher]